Nucleic Acid Interaction Analysis
نویسنده
چکیده
The concept of double stranded DNA forming triple helical complexes with a third strand of nucleic acids via so called Hoogsteenhydrogen bonds has been well established for the last 50 years. However, the topic reentered the big stage with recent publications pointing to an existence of these structures in vivo, highlighting their putative role in chromatin organization and transcriptional regulation in a non-coding RNA mediated context. In this study we characterize the formation of such complexes in vitro, using MicroScale Thermophoresis (MST). The study also highlights the high content information of the MST measurements as one important benefit of MicroScale Thermophoresis.
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